Each episode of Destination Truth runs for about 45 minutes and typically features two investigations of paranormal activity such as spirits and cryptozoological creatures. They usually go to the places where the cryptid is reported. They usually use eyewitness reports and go look for evidence of the creature. If evidence is found, it is sent to be analyzed.
The show can be both scary and funny because of the jokes that the team are known to make as they travel through the places they visit.